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Ub-AMC
a quenched, fluorescent substrate for ubiquitin proteases
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Ub-Rh110Gly
a quenched, fluorescent substrate for ubiquitin proteases
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Ub-VME
a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs)
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Ub-FP
a fluorescence polarization assay reagent based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to ubiquitin via a native isopeptide bond with the lysine side-chain
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NEDD8-FP
a fluorescence polarization assay reagent based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to Nedd8 via a native isopeptide bond with the lysine side-chain
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Ub-aminoluciferin
Ub-aminoluciferin (UbiQ-036) is a DUB activity assay reagent based on ubiquitin that is C-terminally functionalized with 6-aminoluciferin.
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K119 Ub-H2AX(115-143)-FP
a class II fluorescence polarization assay reagent based on the peptide sequence 115 – 143 of the histone protein H2A-X, which is monoubiquitinated on Lys119
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Ub-yH2AX-Ser140(PO4)-FP
a class II fluorescence polarization assay reagent based on the peptide sequence 115 – 143 of the histone protein γH2A-X, which is monoubiquitinated on Lys119 and phosphorylated on Ser140
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(Biotin-Ahx-Ub)-(DNP-Ahx-Ub) K48
a native K48-linked di-ubiquitin which contains a biotin on the N-terminus of the proximal ubiquitin and N-(2,4-dinitrophenyl) (DNP) group on the N-terminus of the distal ubiquitin
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linear di-ubiquitin
a native linear (i.e. M1) linked di-ubiquitin
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ISG15-FP
a fluorescence polarization assay reagent for deISGylating enzymes, based on a TAMRA modified Lys-Gly sequence, linked to mouse ISG15
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Biotin-ANP-Ub-PA
a potent and specific inhibitor of deubiquitylating enzymes (DUBs), labeled on the N-terminus with biotin and containing the UV cleavable 3-amino-3-(2-nitrophenyl)propanoic acid (ANP) linker, allowing for UV mediated cleavage of…
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