custom ubiquitinated peptides

Our UbiQ-PEP™ service provides monoubiquitinated peptides with a range of modifications to meet your research needs. Peptides up to 20 amino acids in length are site-selectively monoubiquitinated via a native isopeptide link using our Ub ligation technology. [1,2]

applications:

  • epitope mapping (e.g. with deubiquitinating enzymes)
  • protein structure-function analysis
  • pull-down experiments [3]
  • ligand for X-ray analysis of proteins

specifications and available options with UbiQ-PEP™ service

peptide length

up to 20 amino acids

 
ubiquitinated lysinesite-selectively monoubiquitinated via a native isopeptide link 
minimal manufacturing scale1 mg 
purity>95% by HPLC 
n-terminal modificationsacetyl, biotin, TAMRA, 5-carboxyRh110 
quality controlLC-MS 
standard delivery time4 to 8 weeks

want to discuss your project and/or receive a detailed quote?

Please contact us at sales@ubiqbio.com

literature

  1. El Oualid et al. “Chemical synthesis of ubiquitin, ubiquitin-based probes, and diubiquitin”. Angew Chem Int Ed. 201049, 10149. 
    http://www.ncbi.nlm.nih.gov/pubmed/21117055/
  2. F. El Oualid & H. Ovaa. “Thiolysine and selenolysine building blocks and their use in site- and chemoselective modification of peptides and proteins. 2009. U.S. Patent U58729009. PCT/NL2010/050277, US61/176,616, EP09160430.6
  3. Fradet-Turcotte et al. “53BP1 is a reader of the DNA-damage-induced H2A Lys 15 ubiquitin mark”. Nature 2013, 499, 50.
    http://www.ncbi.nlm.nih.gov/pubmed/23760478