Ub-AMC

a quenched, fluorescent substrate for ubiquitin proteases

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Weight 0.05 kg
aliquot size

bulk

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Applications

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sample preparation

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regulatory statement

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Description

Ub-AMC (UbiQ-001) is a quenched, fluorescent substrate for ubiquitin proteases. Cleavage of the amide bond between the C-terminal glycine of ubiquitin and 7-amido-4-methylcoumarin (AMC) results in an increase in AMC fluorescence at 460 nm (Exc. 380 nm).

Additional information

Weight 0.05 kg
aliquot size

bulk

,

Applications

,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Dang, L.C., et al. Kinetic and mechanistic studies on the hydrolysis of ubiquitin C-terminal 7-amido-4-methylcoumarin by deubiquitinating enzymes. Biochemistry. 37, 1868-1879 (1998). http://www.ncbi.nlm.nih.gov/pubmed/9485312