Ub-Rh110Gly

a quenched, fluorescent substrate for ubiquitin proteases

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Weight 0.05 kg
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Description

Ubiquitin-Rhodamine110Gly (UbiQ-002) is a quenched, fluorescent substrate for ubiquitin proteases. Cleavage of the amide bond between the C-terminal glycine of ubiquitin and rhodamine110Gly (Rh110Gly) results in an increase in fluorescence at 535 nm (Exc. 485 nm).

Additional information

Weight 0.05 kg
aliquot size

bulk

, , ,

Applications

,

target

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Tirat, A., et al. Synthesis and characterization of fluorescent ubiquitin derivatives as highly sensitive substrates for the deubiquitinating enzymes UCH-L3 and USP-2. Anal. Biochem. 343, 244-255 (2005). http://www.ncbi.nlm.nih.gov/pubmed/15963938

Hassiepin, U., et al. A sensitive fluorescence intensity assay for deubiquitinating proteases using ubiquitin-rhodamine 110-glycine as substrate. Anal. Biochem. 371, 201-207 (2007). http://www.ncbi.nlm.nih.gov/pubmed/17869210