Ub-VME
a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs)
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| Applications | Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling, DUB activity profiling, Compound library screening, Target inhibitor profiling |
| target | DUB, Nedd8, SUMO, ISG15, Ubl proteases |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | upon arrival, powder at −20°C and solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€200.00
- Description
- Additional information
- references
Description
Ub-VME (UbiQ-005) is a potent, irreversible and specific inhibitor of deubiquitinating enzymes (DUBs). It inhibits the hydrolysis of poly-ubiquitin chains on substrate proteins in vitro and thus enhances poly-Ub chain accumulation. UbiQ-005 functions as an activity-based DUB probe and can also be used for structural biology studies of DUB-Ub complexes
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| Applications | Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling, DUB activity profiling, Compound library screening, Target inhibitor profiling |
| target | DUB, Nedd8, SUMO, ISG15, Ubl proteases |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | upon arrival, powder at −20°C and solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
Galardy, P., et al. Mechanism-based proteomics tools based on ubiquitin and ubiquitin-like proteins: crystallography, activity profiling, and protease identification. Methods in Enzymology. 399, 120-131 (2005). http://www.ncbi.nlm.nih.gov/pubmed/16338352
de Jong, A., et al. Ubiquitin-based probes prepared by total synthesis to profile the activity of deubiquitinating enzymes. ChemBiochem 13, 2251-2258 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/23011887