Ub-yH2AX-Ser140(PO4)-FP

a class II fluorescence polarization assay reagent based on the peptide sequence 115 – 143 of the histone protein γH2A-X, which is monoubiquitinated on Lys119 and phosphorylated on Ser140

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code UbiQ-039 Category
product UbiQ-039 Category

Additional information

Weight 0.005 kg
aliquot size

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For detailed sample preparation see product sheet.

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Description

UbiQ-039 (Ub-yH2AX-Ser140(PO4)-FP) is a class II fluorescence polarization assay reagent based on the peptide sequence 115 – 143 of the histone protein γH2A-X, which is monoubiquitinated on Lys119 and phosphorylated on Ser140. The peptide is modified on the N-terminus with a 5-carboxytetramethylrhodamine, a phosphate group on Ser140 and conjugated at Lys119 to Ub via a native isopeptide bond.

Additional information

Weight 0.005 kg
aliquot size

Applications

,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Tirat, A., et al. Synthesis and characterization of fluorescent ubiquitin derivatives as highly sensitive substrates for the deubiquitinating enzymes UCH-L3 and USP-2. Anal. Biochem. 343, 244-255 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/15963938

Huang, X. & Aulabaugh A. Application of fluorescence polarization in HTS assays. Methods in Molecular Biology 565, 127-143 (2009).
http://www.ncbi.nlm.nih.gov/pubmed/19551360

Levine, L.M., et al. Measurement of specific protease activity utilizing fluorescence polarization. Anal. Biochem. 247, 83-88 (1997).
http://www.ncbi.nlm.nih.gov/pubmed/9126375

Geurink, P.P., et al. A general chemical ligation approach towards isopeptide-linked ubiquitin and ubiquitin-like assay reagents. ChemBiochem 13, 293-297 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/22213387