Biotin-ANP-Ub-PA

a potent and specific inhibitor of deubiquitylating enzymes (DUBs), labeled on the N-terminus with biotin and containing the UV cleavable 3-amino-3-(2-nitrophenyl)propanoic acid (ANP) linker, allowing for UV mediated cleavage of the biotin tag

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Description

Biotin-ANP-Ub-PA (UbiQ-077) is potent and specific inhibitor of deubiquitylating enzymes (DUBs). It is labeled on the N-terminus with biotin and contains the UV cleavable 3-amino-3-(2-nitrophenyl)propanoic acid (ANP) linker, allowing for UV mediated cleavage of the biotin tag.

This activity probe can be used for activity profiling experiments and determining DUB inhibitor specificity. It has two unique capabilities:

1. it forms a covalent linkage with (the active site Cys residue of) a DUB that can be cleaved by acid treatment (5% aq. TFA), allowing for proteomic analyses.

2. it targets all three major DUB families: UCH, USP and OTU.

Rodenko et al. Nat Prot 2006, 1, 1120
https://www.ncbi.nlm.nih.gov/pubmed/17406393

Ekkebus et al. J. Am. Chem. Soc. 2013, 135, 2867
https://www.ncbi.nlm.nih.gov/pubmed/23387960

Sommer et al. Bioorg. Med. Chem. 2013, 21, 2511
https://www.ncbi.nlm.nih.gov/pubmed/23535560