
(Biotin-Ahx-Ub)-(DNP-Ahx-Ub) K48
a native K48 linked diubiquitin which contains a biotin on the N-terminus of the proximal ubiquitin and a dinitrophenyl group on the N-terminus of the distal ubiquitin
Additional information
Weight | 0.05 kg |
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aliquot size | |
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shipping | |
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storage | Upon arrival, powder at −20°C; solution at −80°C. Please avoid multiple freeze/thaw cycles. |
sample preparation | For detailed sample preparation see product sheet. |
regulatory statement |
€150.00
- Description
- Additional information
- references
Description
UbiQ-056 is a native K48 linked di-Ub which is equiped with a biotin on the N-terminus of the proximal ubiquitin and a dinitrophenyl group on the N-terminus of the distal ubiquitin (DNP= N-(2,4- dinitrophenyl)-6-aminohexanoic Acid). Both tags are separated from the N-terminus by an additional aminohexanoic acid (Ahx) linker. UbiQ-056 is designed to be used as substrate for proteases that cleave the isopeptide linkage between two ubiquitin molecules.This product is formed by chemical ligation.
Additional information
Weight | 0.05 kg |
---|---|
aliquot size | |
Applications | |
target | |
source | |
shipping | |
purity | |
molecular weight | |
storage | Upon arrival, powder at −20°C; solution at −80°C. Please avoid multiple freeze/thaw cycles. |
sample preparation | For detailed sample preparation see product sheet. |
regulatory statement |
F. El Oualid et al.
El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055
R. A. Horton et al.
Horton, R.A., et al. A substrate for deubiquitinating enzymes based on time-resolved fluorescence resonance energy transfer between terbium and yellow fluorescent protein. Anal. Biochem. 360, 138-143 (2007).
http://www.ncbi.nlm.nih.gov/pubmed/17118327
I. H. Engels et al.
Engels, I.H., et al. A time-resolved fluorescence resonance energy transfer-based assay for DEN1 peptidase activity. Anal. Biochem. 390, 85-87 (2009).
http://www.ncbi.nlm.nih.gov/pubmed/19328766