(Biotin-Ahx-Ub)-(DNP-Ahx-Ub) K48

a native K48 linked diubiquitin which contains a biotin on the N-terminus of the proximal ubiquitin and a dinitrophenyl group on the N-terminus of the distal ubiquitin

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code UbiQ-056 Category
product UbiQ-056 Category

Additional information

Weight 0.05 kg
aliquot size

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regulatory statement

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Description

UbiQ-056 is a native K48 linked di-Ub which is equiped with a biotin on the N-terminus of the proximal ubiquitin and a dinitrophenyl group on the N-terminus of the distal ubiquitin (DNP= N-(2,4- dinitrophenyl)-6-aminohexanoic Acid). Both tags are separated from the N-terminus by an additional aminohexanoic acid (Ahx) linker. UbiQ-056 is designed to be used as substrate for proteases that cleave the isopeptide linkage between two ubiquitin molecules.This product is formed by chemical ligation.

Additional information

Weight 0.05 kg
aliquot size

Applications

,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

F. El Oualid et al.
El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055

R. A. Horton et al.
Horton, R.A., et al. A substrate for deubiquitinating enzymes based on time-resolved fluorescence resonance energy transfer between terbium and yellow fluorescent protein. Anal. Biochem. 360, 138-143 (2007).
http://www.ncbi.nlm.nih.gov/pubmed/17118327

I. H. Engels et al.
Engels, I.H., et al. A time-resolved fluorescence resonance energy transfer-based assay for DEN1 peptidase activity. Anal. Biochem. 390, 85-87 (2009).
http://www.ncbi.nlm.nih.gov/pubmed/19328766