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Ub-VME
a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs)
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Ub-FP
a fluorescence polarization assay reagent based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to ubiquitin via a native isopeptide bond with the lysine side-chain
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NEDD8-FP
a fluorescence polarization assay reagent based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to Nedd8 via a native isopeptide bond with the lysine side-chain
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SUMO1-FP
a fluorescence polarization assay reagent for de-SUMOylating enzyme, based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to (6His tagged) SUMO-1 via a native isopeptide bond with the…
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SUMO2-FP
a fluorescence polarization assay reagent for de-SUMOylating enzyme, based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to (6His tagged) SUMO-2 via a native isopeptide bond with the…
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(Biotin-Ahx-Ub)-(DNP-Ahx-Ub) K48
a native K48-linked di-ubiquitin which contains a biotin on the N-terminus of the proximal ubiquitin and N-(2,4-dinitrophenyl) (DNP) group on the N-terminus of the distal ubiquitin
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ISG15-FP
a fluorescence polarization assay reagent for deISGylating enzymes, based on a TAMRA modified Lys-Gly sequence, linked to mouse ISG15
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Cy5-Ub-Dha
an activity-based probe for Ub E1, E2 and (HECT/RBR) E3 ligases, labeled with a Cy5 dye on the N-terminus
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(Biotin-Ahx-Ub)-(Cys-Ahx-Ub) K48 linked
a native K48 linked di-ubiquitin, modified with biotin on the N-terminus of the distal Ub and cysteine on the N-terminus of the proximal Ub
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5-carboxyRh110-Nedd8-Dha
an activity-based probe for Nedd8 E1, E2 and E3 enzymes, labeled with cRh110 on the N-terminus
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Ub-Rh110MP
a quenched, fluorescent substrate for ubiquitin proteases, releasing a brighter Rh110MP dye upon DUB cleavage than UbRh110Gly
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Ac-ISG15prox-Rh110MP
a quenched, fluorescent substrate for ISG15 proteases based on the proximal part of (mouse) ISG15
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