SUMO1-FP

a fluorescence polarization assay reagent for de-SUMOylating enzyme, based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to (6His tagged) SUMO-1 via a native isopeptide bond with the lysine side-chain

productsheet

code UbiQ-020 Category
product UbiQ-020 Category

Additional information

Weight 0.05 kg
aliquot size

Applications

,

target

source

human sequence, semi-synthetic

shipping

purity

>95%

molecular weight

storage

sample preparation

upon arrival, powder at −20°C, solution at −80°C. Protect from light and avoid multiple freeze/thaw cycles.

regulatory statement

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300.00

Description

SUMO1-FP (UbiQ-020) is a fluorescence polarization assay reagent for de-SUMOylating enzymes which is based on a 5-carboxytetramethylrhodamine (TAMRA, exc 550 nm, emi 590 nm) modified Lys-Gly sequence that is linked to (6His tagged) SUMO-1 via a native isopeptide bond with the lysine side-chain.

A typical substrate concentration is 100 nM. Effective concentrations of deSUMOylating enzymes can range from 0.01-10 nM but depend on specific assay conditions and method of detection.

UbiQ-020 is effectively processed by SENP1.

Additional information

Weight 0.05 kg
aliquot size

Applications

,

target

source

human sequence, semi-synthetic

shipping

purity

>95%

molecular weight

storage

sample preparation

upon arrival, powder at −20°C, solution at −80°C. Protect from light and avoid multiple freeze/thaw cycles.

regulatory statement