Cy5-Ub-Dha

an activity-based probe for Ub E1, E2 and (HECT/RBR) E3 ligases, labeled with a Cy5 dye on the N-terminus

productsheet

code UbiQ-104 Category
product UbiQ-104 Category

Additional information

Weight 0.005 kg
aliquot size

bulk

Applications

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purity

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storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Clear

350.00

Description

Cy5-Ub-Dha (UbiQ-104) is an activity-based probe for Ub E1, E2 and (HECT/RBR) E3 ligases. It is based on the Ub sequence in which the C-terminal Gly76 has been replaced by a dehydroalanine (Dha) residue. The N-terminus is labeled with a Cy5 tag. It has been prepared by total chemical synthesis and is therefore well-defined in terms of Cy5 site. UbiQ-104 is processed in a native manner by Ub E1, E2 and (HECT/RBR) E3 ligases and during this process it forms an electrophilic intermediate that can react with the active site Cys residue of the E1, E2 and (HECT/RBR) E3 enzyme, thereby creating a covalent bond.

Additional information

Weight 0.005 kg
aliquot size

bulk

Applications

, ,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Mulder et al., A cascading activity-based probe sequentially targets E1–E2–E3 ubiquitin enzymes. Nature Chem Bio (2016) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5108872/