K33 linked di-ubiquitin

a native K33 linked di-ubiquitin

productsheet

code UbiQ-017 Category
product UbiQ-017 Category

Additional information

Weight 0.025 kg
aliquot size

Applications

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target

source

shipping

purity

molecular weight

storage

Powder at −20°C; solution at −80°C. Please avoid multiple freeze/thaw cycles.

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

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Description

UbiQ-017 is native K33 linked di-ubiquitin which can be used as a substrate for proteases that cleave the isopeptide linkage between two ubiquitin proteins. It can also be used to investigate mechanism of binding and recognition by proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs (UIMs). This product is formed by chemical ligation.

Additional information

Weight 0.025 kg
aliquot size

Applications

,

target

source

shipping

purity

molecular weight

storage

Powder at −20°C; solution at −80°C. Please avoid multiple freeze/thaw cycles.

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055

Faesen, A.C., et al. The Differential Modulation of USP Activity by Internal Regulatory Domains, Interactors and Eight Ubiquitin Chain Types. Chem. Biol. 18, 1550-1561 (2011).
http://www.ncbi.nlm.nih.gov/pubmed/22195557

Licchesi, J.D., et al. An ankyrin-repeat ubiquitin-binding domain determines TRABID’s specificity for atypical ubiquitin chains. Nat. Struct. Mol. Biol. 19, 62-71 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/22157957

Dikic, I., et al. Ubiquitin-binding domains – from structures to functions. Nat. Rev. Mol. Cell. Biol. 10, 659-671 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/19773779