reagents

SUMO1-FP

a fluorescence polarization assay reagent for de-SUMOylating enzyme, based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to (6His tagged) SUMO-1 via a native isopeptide bond with the…

UbiQ-020

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SUMO2-FP

a fluorescence polarization assay reagent for de-SUMOylating enzyme, based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to (6His tagged) SUMO-2 via a native isopeptide bond with the…

UbiQ-021

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5-TAMRA-Lys-Gly-OH

a fluorescence polarization HTS control reagent that can be used to make a concentration calibration curve and determine the grating factor (G)

productsheet

UbiQ-023

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Ub-FANCD2-FP

a class II fluorescence polarization DUB activity assay reagent based on the peptide sequence 557– 565 of FANCD2, a DNA repair protein which is monoubiquitinated on Lys561

UbiQ-029

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K13 Ub-PTEN(5-21)-FP

a class II fluorescence polarization DUB activity assay reagent based on the peptide sequence 5 – 21 of PTEN, which is monoubiquitinated on Lys13 and Lys289

UbiQ-030

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K48 linked di-ubiquitin

a native K48 linked di-ubiquitin

productsheet

UbiQ-033

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K63 linked di-ubiquitin

a native K63 linked di-ubiquitin

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UbiQ-034

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HA-Ahx-Ahx-Ub-VME

a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs), labeled with HA on the N-terminus

productsheet

UbiQ-035

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Ub-aminoluciferin

Ub-aminoluciferin (UbiQ-036) is a DUB activity assay reagent based on ubiquitin that is C-terminally functionalized with 6-aminoluciferin.

productsheet

UbiQ-036

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K119 Ub-H2AX(115-143)-FP

a class II fluorescence polarization assay reagent based on the peptide sequence 115 – 143 of the histone protein H2A-X, which is monoubiquitinated on Lys119

UbiQ-038

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Ub-yH2AX-Ser140(PO4)-FP

a class II fluorescence polarization assay reagent based on the peptide sequence 115 – 143 of the histone protein γH2A-X, which is monoubiquitinated on Lys119 and phosphorylated on Ser140

UbiQ-039

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K382 Ub-p53(368-389)-FP

UbiQ-041 is a class II fluorescence polarization HTS reagent based on the C-terminal p53 peptide sequence 368-389, conjugated at Lys382 to Ub via a native isopeptide bond.

UbiQ-041

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