Ub-FANCD2-FP

a class II fluorescence polarization DUB activity assay reagent based on the peptide sequence 557– 565 of FANCD2, a DNA repair protein which is monoubiquitinated on Lys561

productsheet

code UbiQ-029 Category Tags , ,
product UbiQ-029 Category Tags , ,

Additional information

Weight 0.005 kg
aliquot size

Applications

,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Clear

300.00

Description

UbiQ-029 (Ub-FANCD2-FP) is a class II fluorescence polarization DUB activity assay reagent based on the peptide sequence 557– 565 of FANCD2, a DNA repair protein which is monoubiquitinated on Lys561. The peptide is modified on the N-terminus with a 5-carboxytetramethylrhodamine and conjugated at Lys561to ubiquitin via a native isopeptide bond.

Additional information

Weight 0.005 kg
aliquot size

Applications

,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Tirat, A., et al. Synthesis and characterization of fluorescent ubiquitin derivatives as highly sensitive substrates for the deubiquitinating enzymes UCH-L3 and USP-2. Anal. Biochem. 343, 244-255 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/15963938

Huang, X. & Aulabaugh A. Application of fluorescence polarization in HTS assays. Methods in Molecular Biology 565, 127-143 (2009).
http://www.ncbi.nlm.nih.gov/pubmed/19551360

Levine, L.M., et al. Measurement of specific protease activity utilizing fluorescence polarization. Anal. Biochem. 247, 83-88 (1997).
http://www.ncbi.nlm.nih.gov/pubmed/9126375

Geurink, P.P., et al. A general chemical ligation approach towards isopeptide-linked ubiquitin and ubiquitin-like assay reagents. ChemBiochem 13, 293-297 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/22213387

Sheng, Y., et al. Molecular recognition of p53 and MDM2 by USP7/HAUSP. Nat. Struct. Mol. Biol. 13, 285-291 (2006).
http://www.ncbi.nlm.nih.gov/pubmed/16474402