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Ub-AMC
a quenched, fluorescent substrate for ubiquitin proteases
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Ub-Rh110Gly
a quenched, fluorescent substrate for ubiquitin proteases
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TAMRA-Ub
a fluorescent ubiquitin, labeled with TAMRA on the N-terminus
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Ub-FP
a fluorescence polarization assay reagent based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to ubiquitin via a native isopeptide bond with the lysine side-chain
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5-TAMRA-Lys-Gly-OH
a fluorescence polarization HTS control reagent that can be used to make a concentration calibration curve and determine the grating factor (G)
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Ub-FANCD2-FP
a class II fluorescence polarization DUB activity assay reagent based on the peptide sequence 557– 565 of FANCD2, a DNA repair protein which is monoubiquitinated on Lys561
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Ub-aminoluciferin
Ub-aminoluciferin (UbiQ-036) is a DUB activity assay reagent based on ubiquitin that is C-terminally functionalized with 6-aminoluciferin.
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K382 Ub-p53(368-389)-FP
UbiQ-041 is a class II fluorescence polarization HTS reagent based on the C-terminal p53 peptide sequence 368-389, conjugated at Lys382 to Ub via a native isopeptide bond.
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K382 Ub-p53(357-389)-FP
UbiQ-042 is a class II fluorescence polarization HTS reagent based on the C-terminal p53 peptide sequence 357-389, conjugated at Lys382 to Ub via a native isopeptide bond.
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Ub pSer65
ubiquitin, phosphorylated on Ser65, activating Parkin E3 ligase mediated ubiquitination
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Biotin-Ahx-Ub
Ubiquitin, labeled on the N-terminus with biotin
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Biotin-Ahx-Ub pSer 65
ubiquitin, phosphorylated on Ser65, labeled on the N-terminus with biotin, activating Parkin E3 ligase mediated ubiquitination
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