Ubiquitin, labeled on the N-terminus with biotin


code UbiQ-090 Category Tags ,
product UbiQ-090 Category Tags ,
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UbiQ-090 is (synthetic) ubiquitin labeled on the N-terminus with biotin; an aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and Ubiquitin protein for efficient access of biotin binding entities. It has been prepared by total chemical synthesis and is therefore well-defined in terms of biotinylation site. UbiQ-090 can be used alone or in combination with unlabeled Ubiquitin.

El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).

Wang, T., et al. Biotin-ubiquitin tagging of mammalian proteins in Escherichia coli. Protein Expr. Purif. 30, 140-149 (2003).