Biotin-Ahx-Ub pSer 65

ubiquitin, phosphorylated on Ser65, labeled on the N-terminus with biotin, activating Parkin E3 ligase mediated ubiquitination

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code UbiQ-091 Category Tags ,
product UbiQ-091 Category Tags ,

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Weight 0.005 kg
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Description

UbiQ-091 is ubiquitin that is phosphorylated on Ser65, a ubiquitin variant which has been shown to activate Parkin E3 ligase mediated ubiquitination. It is labeled on the N-terminus with biotin; an aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and Ub protein for efficient access of biotin binding entities. It has been made by total chemical synthesis and is therefore well-defined in terms of biotin and pSer site and incorporation efficiency (100%).

Additional information

Weight 0.005 kg
aliquot size

bulk size

Applications

, , ,

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Kane et al. J Cell Biol 2014, 205, 143-153.
https://www.ncbi.nlm.nih.gov/pubmed/24751536

Kazlauskaite et al. Biochem J 2014, 460, 127-139.
https://www.ncbi.nlm.nih.gov/pubmed/24660806

Kondapalli et al. Open Biol 2012, 2, 120080.
https://www.ncbi.nlm.nih.gov/pubmed/22724072

Koyano et al. Nature 2014, 510, 162-166.
https://www.ncbi.nlm.nih.gov/pubmed/24784582

V. Sauve and K. Gehring Cell Res 2014, 24, 1025.
https://www.ncbi.nlm.nih.gov/pubmed/24946738

Spratt et al. Nat Commun 2013, 4, 1983.
https://www.ncbi.nlm.nih.gov/pubmed/23770917

Trempe et al. Science 2013, 340, 1451.
https://www.ncbi.nlm.nih.gov/pubmed/23661642

T. Wauer and D. Komander EMBO J 2013, 32, 2099-2112.
https://www.ncbi.nlm.nih.gov/pubmed/23727886

Yamamoto et al. J Biol Chem 2005, 280, 3390-3399.
https://www.ncbi.nlm.nih.gov/pubmed/15557340