TAMRA-Ub

a fluorescent ubiquitin, labeled with TAMRA on the N-terminus

productsheet

code UbiQ-003 Category Tags ,
product UbiQ-003 Category Tags ,

Additional information

Weight 0.1 kg
aliquot size

Applications

target

source

human sequence, synthetic

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

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350.00

Description

TAMRA-Ub (UbiQ-003) is fluorescent ubiquitin labeled on the N-terminus with TAMRA (5- tetramethylrhodamine, exc 550 nm, emi 590 nm). It has been prepared by total chemical synthesis and allows detection of ubiquitylation by in-gel fluorescence. This direct and more sensitive read-out gives more distinct labeling patterns than immunoblotting and does not suffer from background labeling due to cross-reactivity (as seen sometimes with antibody based detection).

Additional information

Weight 0.1 kg
aliquot size

Applications

target

source

human sequence, synthetic

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055

de Jong, A., et al. Ubiquitin-based probes prepared by total synthesis to profile the activity of deubiquitinating enzymes. ChemBiochem 13, 2251-2258 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/23011887

Smit, J.J. et al. Target specificity of the E3 ligase LUBAC for ubiquitin and NEMO relies on different minimal requirements. J. Biol. Chem. 288, 31728-31737 (2013). http://www.ncbi.nlm.nih.gov/pubmed/24030825