UbiQ Assay

K720 Nedd8-cullin1 (715-725)-FP

a class II fluorescence polarization HTS reagent based on the cullin-1 (715-725) polypeptide, modified on the N-terminus with a 5-carboxytetramethylrhodamine and mononeddylated at K720 via a native isopeptide bond

UbiQ-096

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(Biotin-Ahx-Ub)-(Cys-Ahx-Ub) K48 linked

a native K48 linked di-ubiquitin, modified with biotin on the N-terminus of the distal Ub and cysteine on the N-terminus of the proximal Ub

UbiQ-119

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Ub-Rh110MP

a quenched, fluorescent substrate for ubiquitin proteases, releasing a brighter Rh110MP dye upon DUB cleavage than UbRh110Gly

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UbiQ-126

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Ac-ISG15prox-Rh110MP

a quenched, fluorescent substrate for ISG15 proteases based on the proximal part of (mouse) ISG15

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UbiQ-127

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5-carboxyRh110-SUMO2

human SUMO2 sequence (C48S), labeled with cRh110 on the N-terminus

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UbiQ-134

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Mca-KKVAPYPME-Dap(Dnp)–NH2

an LF2 peptide based fluorogenic proteasome assay reagent for monitoring 26S proteasome, or ClpP protease activity

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UbiQ-141

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Biotin-Ahx-H2B(106-125)

an H2B(106-125) peptide, labeled on the N-terminus with biotin

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UbiQ-151

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Ub photoLeu8

a crosslinking reagent based on ubiquitin in which Leu8 is replaced by the photoreactive analog photoleucine (photoLeu)

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UbiQ-153

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Ub photoLeu73

a crosslinking reagent based on ubiquitin in which Leu73 is replaced by the photoreactive analog photoleucine (photoLeu)

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UbiQ-154

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LRRASLG-GG-H2B(113-125)

an H2B(113-125) peptide which is modified on the N-terminus with a PKA (cAMP- dependent Protein Kinase) sequence: LRRASLG

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UbiQ-333

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custom assay reagents

Ubiquitin(-like) (e.g. Ub, Nedd8, SUMO, etc.) assay reagents with various modifications (unnatural amino acids, peptides, tags, etc.)

UbiQ-C01

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DUB assay explorer panel

a panel of 4 deubiquitylating enzyme (DUB) activity assay reagents (25 μg each) prepared by total chemical synthesis

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UbiQ-L03

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