Ub-Rh110MP

a quenched, fluorescent substrate for ubiquitin proteases, releasing a brighter Rh110MP dye upon DUB cleavage than UbRh110Gly

productsheet

code UbiQ-126 Category Tag
product UbiQ-126 Category Tag

Additional information

Weight 0.005 kg
aliquot size

bulk

, ,

Applications

,

target

source

human sequence, synthetic

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

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200.00

Description

Ub-Rh110MP (UbiQ-126) is a quenched, fluorescent substrate for deubiquitylases (DUBs). Cleavage of the amide bond between Gly76 of ubiquitin and the Rhodamine110 moiety releases the highly fluorescent Rh110-morpholinecarbonyl which exhibits a higher fluorescence intensity than the classical Rh110Gly fluorophore of Ub-Rh110Gly (UbiQ-002). UbiQ-126 is prepared by total chemical synthesis.

Additional information

Weight 0.005 kg
aliquot size

bulk

, ,

Applications

,

target

source

human sequence, synthetic

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Hassiepin, U., et al. A sensitive fluorescence intensity assay for deubiquitinating proteases using ubiquitin-rhodamine 110-glycine as substrate. Anal. Biochem. 371, 201-207 (2007).
http://www.ncbi.nlm.nih.gov/pubmed/17869210

El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055

Lavis, L.D., et al. Fluorogenic label for biomolecular imaging. ACS Chem. Biol. 1, 252-260 (2006).
http://www.ncbi.nlm.nih.gov/pubmed/17163679

Terentyeva, T.G., et al. Morpholinecarbonyl-Rhodamine 110 based substrates for the determination of protease activity with accurate kinetic parameters. Bioconj. Chem. 22, 1932-1938 (2011).
http://www.ncbi.nlm.nih.gov/pubmed/21905728