K720 Nedd8-cullin1 (715-725)-FP

a class II fluorescence polarization HTS reagent based on the cullin-1 (715-725) polypeptide, modified on the N-terminus with a 5-carboxytetramethylrhodamine and mononeddylated at K720 via a native isopeptide bond

productsheet

code UbiQ-096 Category
product UbiQ-096 Category

Additional information

Weight 0.005 kg
aliquot size

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purity

molecular weight

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sample preparation

For detailed sample preparation see product sheet.

regulatory statement

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300.00

Description

UbiQ-096 is a class II fluorescence polarization HTS reagent based on the cullin-1 (715-725) polypeptide. The peptide is modified on the N-terminus with a 5-carboxytetramethylrhodamine and mononeddylated at K720 via a native isopeptide bond. It can be used as a substrate for Nedd8 proteases. This product is formed by chemical ligation. See reference 4 in the product sheet (open access) for full experimental assay details.

Additional information

Weight 0.005 kg
aliquot size

Applications

,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Tirat, A., et al. Synthesis and characterization of fluorescent ubiquitin derivatives as highly sensitive substrates for the deubiquitinating enzymes UCH-L3 and USP-2. Anal. Biochem. 343, 244-255 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/15963938

Huang, X. & Aulabaugh A. Application of fluorescence polarization in HTS assays. Methods in Molecular Biology 565, 127-143 (2009).
http://www.ncbi.nlm.nih.gov/pubmed/19551360

Levine, L.M., et al. Measurement of specific protease activity utilizing fluorescence polarization. Anal. Biochem. 247, 83-88 (1997).
http://www.ncbi.nlm.nih.gov/pubmed/9126375

Geurink, P.P., et al. A general chemical ligation approach towards isopeptide-linked ubiquitin and ubiquitin-like assay reagents. ChemBiochem 13, 293-297 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/22213387

Faesen, A.C., et al. The Differential Modulation of USP Activity by Internal Regulatory Domains, Interactors and Eight Ubiquitin Chain Types. Chem. Biol. 18, 1550-1561 (2011).
http://www.ncbi.nlm.nih.gov/pubmed/22195557