UbiQ Assay
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Ub-AMC
a quenched, fluorescent substrate for ubiquitin proteases
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Ub-Rh110Gly
a quenched, fluorescent substrate for ubiquitin proteases
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Ub-FP
a fluorescence polarization assay reagent based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to ubiquitin via a native isopeptide bond with the lysine side-chain
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NEDD8-FP
a fluorescence polarization assay reagent based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to Nedd8 via a native isopeptide bond with the lysine side-chain
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SUMO1-FP
a fluorescence polarization assay reagent for de-SUMOylating enzyme, based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to (6His tagged) SUMO-1 via a native isopeptide bond with the…
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SUMO2-FP
a fluorescence polarization assay reagent for de-SUMOylating enzyme, based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to (6His tagged) SUMO-2 via a native isopeptide bond with the…
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5-TAMRA-Lys-Gly-OH
a fluorescence polarization HTS control reagent that can be used to make a concentration calibration curve and determine the grating factor (G)
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Ub-FANCD2-FP
a class II fluorescence polarization DUB activity assay reagent based on the peptide sequence 557– 565 of FANCD2, a DNA repair protein which is monoubiquitinated on Lys561
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K13 Ub-PTEN(5-21)-FP
a class II fluorescence polarization DUB activity assay reagent based on the peptide sequence 5 – 21 of PTEN, which is monoubiquitinated on Lys13 and Lys289
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Ub-aminoluciferin
Ub-aminoluciferin (UbiQ-036) is a DUB activity assay reagent based on ubiquitin that is C-terminally functionalized with 6-aminoluciferin.
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K119 Ub-H2AX(115-143)-FP
a class II fluorescence polarization assay reagent based on the peptide sequence 115 – 143 of the histone protein H2A-X, which is monoubiquitinated on Lys119
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Ub-yH2AX-Ser140(PO4)-FP
a class II fluorescence polarization assay reagent based on the peptide sequence 115 – 143 of the histone protein γH2A-X, which is monoubiquitinated on Lys119 and phosphorylated on Ser140
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