Mca-KKVAPYPME-Dap(Dnp)–NH2 | proteasome activity assay reagent

an LF2 peptide based fluorogenic proteasome assay reagent for monitoring 26S proteasome, or ClpP protease activity

productsheet

code UbiQ-141 Category Tag
product UbiQ-141 Category Tag

Additional information

Weight 0.005 kg
aliquot size

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

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200.00

Description

UbiQ-141, LF2 peptide assay reagent is a long fluorogenic reagent for monitoring ATP dependant protease activity like in the 26S proteasome and ClpP protease. [Jastrab et al.] Because the LF2 peptide is larger than standard tri/tetra peptide based assay reagents, LF2 is not processed by the 20S CP alone and activating factors are required.

UbiQ-141 Key Characteristics:

  • only processed by activated proteasomes [Jastrab et al.]
  • exhibits a much more pronounced stimulation of proteasome activity than tetrapeptide based proteasome activity assay reagents [Jastrab et al.]
  • processed by human and bacterial proteasomes

 

Additional information

Weight 0.005 kg
aliquot size

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Jastrab, J. B., et al.
An adenosine triphosphate-independent proteasome activator contributes to the virulence of Mycobacterium tuberculosis. Proc Natl Acad Sci USA. 127, E1763-E1772 (2015), DOI: 10.1073/pnas.1423319112
https://www.pnas.org/doi/10.1073/pnas.1423319112

Smith DM et al.
Docking of the proteasomal ATPases’ carboxyl termini in the 20S proteasome’s alpha ring opens the gate for substrate entry. Mol Cell. 27 731-744 (2007). DOI: 10.1016/j.molcel.2007.06.033
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2083707/

Smith DM et al.
ATP binding to PAN or the 26S ATPases causes association with the 20S proteasome, gate opening, and translocation of unfolded proteins. Mol Cell. 20 687-698 (2005).
https://doi.org/10.1016/j.molcel.2005.10.019

Bai et al.
Structural analysis of the dodecameric proteasome activator PafE in Mycobacterium tuberculosis. Proc. Natl. Acad. Sci. USA. 127, E1983-1992 (2015) DOI: 10.1073/pnas.1512094113
https://www.pnas.org/doi/abs/10.1073/pnas.1512094113