A cascading activity-based probe sequentially targets E1–E2–E3 ubiquitin enzymes.

Monique P C Mulder, Katharina Witting, Ilana Berlin, Jonathan N Pruneda, Kuen-Phon Wu, Jer-Gung Chang, Remco Merkx, Johanna Bialas, Marcus Groettrup, Alfred C O Vertegaal, Brenda A Schulman, David Komander, Jacques Neefjes, Farid El Oualid* & Huib Ovaa*.

Nature Chemical Biology, 16 May 2016, Advance Online Publication, DOI: 10.1038/NCHEMBIO.2084