Ub-Dha

an activity based probe for Ub E1, E2 and (HECT/RBR) E3 ligases

productsheet

code UbiQ-101 Category
product UbiQ-101 Category

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Weight 0.005 kg
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Description

UbiQ-101 (Ub-Dha, Dha= dehydroalanine) is a first of its kind activity based probe for Ub E1, E2 and (HECT/RBR) E3 ligases [Mulder et al. 2016]. It is based on the Ub sequence in which the C-terminal Gly76 has been replaced by a dehydroalanine residue. It is processed in a native manner by Ub E1, E2 and (HECT/RBR) E3 ligases and during this process it forms an electrophilic intermediate that can react with the active site Cys residue of the E1, E2 and (HECT/RBR) E3 enzyme, thereby creating a covalent bond (see figure).

Additional information

Weight 0.005 kg
bulk

Applications

,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

aliquot size

Mulder et al., A cascading activity-based probe sequentially targets E1–E2–E3 ubiquitin enzymes. Nature Chem Bio (2016) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5108872/