hISG15-FP

Name: hISG15-FP
SKU: UbiQ-287
Availability: InStock

a fluorescence polarization assay reagent for deISGylating enzymes, based on a TAMRA modified Lys-Gly sequence, linked to human ISG15

productsheet

code UbiQ-287 Category activity assay reagents

product UbiQ-287 Category activity assay reagents

Additional information

Weight	0.025 kg
aliquot size	25 µg
Applications	DUB activity profiling, Compound library screening
target	ISG15, Ubl proteases
source	human sequence, semi-synthetic
shipping	22 uM in 50 mM HEPES, 150 mM NaCl, 2 mM DTT
purity	>95%
molecular weight	18.2 kDa
storage	upon arrival, store solution at −80°C. Please protect from light and avoid multiple freeze/thaw cycles.
sample preparation	For detailed sample preparation, please see product sheet.
regulatory statement	For laboratory research use only, not for use in humans.

€300.00

Description
Additional information
references

Description

UbiQ-287 is a fluorescence polarization assay reagent for deISGylating enzymes which is based on a 5-carboxytetramethylrhodamine (TAMRA, exc 550 nm, emi 590 nm) modified Lys-Gly sequence that is linked to human ISG15 via a native isopeptide bond.

Additional information

Weight	0.025 kg
aliquot size	25 µg
Applications	DUB activity profiling, Compound library screening
target	ISG15, Ubl proteases
source	human sequence, semi-synthetic
shipping	22 uM in 50 mM HEPES, 150 mM NaCl, 2 mM DTT
purity	>95%
molecular weight	18.2 kDa
storage	upon arrival, store solution at −80°C. Please protect from light and avoid multiple freeze/thaw cycles.
sample preparation	For detailed sample preparation, please see product sheet.
regulatory statement	For laboratory research use only, not for use in humans.

Tirat, A., et al. Synthesis and characterization of fluorescent ubiquitin derivatives as highly sensitive substrates for the deubiquitinating enzymes UCH-L3 and USP-2. Anal. Biochem. 343, 244-255 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/15963938

Huang, X. & Aulabaugh A. Application of fluorescence polarization in HTS assays. Methods in Molecular Biology 565, 127-143 (2009).
http://www.ncbi.nlm.nih.gov/pubmed/19551360

Levine, L.M., et al. Measurement of specific protease activity utilizing fluorescence polarization. Anal. Biochem. 247, 83-88 (1997).
http://www.ncbi.nlm.nih.gov/pubmed/9126375

Geurink, P.P., et al. A general chemical ligation approach towards isopeptide-linked ubiquitin and ubiquitin-like assay reagents. ChemBiochem 13, 293-297 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/22213387

Basters, A., et al. Molecular characterization of ubiquitin-specific protease 18 reveals substrate specificity for interferon-stimulated gene 15. Febs. J. 281, 1918-1928 (2014).
http://www.ncbi.nlm.nih.gov/pubmed/24533902