K58K(biotin) cRh110-Cys-Ubv(USP7)-PA

a potent, irreversible and specific activity-based probe designed to selectively target USP7, labeled with cRh110 on the N-terminus

productsheet

code UbiQ-314 Category Tags , , ,
product UbiQ-314 Category Tags , , ,

Additional information

Weight 0.005 kg
aliquot size

Applications

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For detailed sample preparation see product sheet.

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350.00

Description

UbiQ-314 is an activity-based probe that is based on a ubiquitin variant designed to selectively target USP7. It contains a C-terminal electrophilic propargyl amide (PA, also sometimes abbreviated as Prg). The PA group forms a covalent linkage with the active site Cys residue of USP7 that can be cleaved by acid treatment (5% aq. TFA), allowing for proteomic analyses. The N-terminus is functionalised with a 5-carboxyrhodamine110 dye (cRh110, λex = 480 nm; λem = 520 nm) allowing for a sensitive and fast (in-gel fluorescence) detection. A Cys residue is introduced between the dye and N-terminus for further modification with thiol-reactive tags. UbiQ-314 is based on Ub with the following mutations: T7D, L8Y, I13R, E34L, Q40N, L69A, and L71A. Residue Lys58 contains a biotin tag which besides detection and binding also serves to increase USP7 selectivity by prohibiting binding of most USPs.

 

Additional information

Weight 0.005 kg
aliquot size

Applications

, , , , , ,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Gjonaj et al. Chem Comm 2019, 55, 5075.
https://pubs.rsc.org/en/content/articlelanding/2019/cc/c9cc00969h

Mandal et al. Angew Chem Int Ed Engl 2021, 60, 7333.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048964/

Ekkebus, R., et al. On terminal alkynes that can react with active-site cysteine nucleophiles in proteases. J. Am. Chem. Soc. 135, 2867-2870 (2013).
http://www.ncbi.nlm.nih.gov/pubmed/23387960

Sommer et al. Bioorg Med Chem 2013, 21, 2511.
https://pubmed.ncbi.nlm.nih.gov/23535560/