K58K(biotin) cRh110-Cys-Ubv(USP7)-PA
activity-based probe designed to selectively target USP7
Additional information
| Weight | 0.005 kg |
|---|---|
| aliquot size | |
| Applications | Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | Powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€350.00
- Description
- Additional information
- references
Description
UbiQ-314 is an activity-based probe that is based on a ubiquitin variant designed to selectively target USP7. It contains a C-terminal electrophilic propargyl amide (PA, also sometimes abbreviated as Prg). The PA group forms a covalent linkage with the active site Cys residue of USP7 that can be cleaved by acid treatment (5% aq. TFA), allowing for proteomic analyses. The N-terminus is functionalised with a 5-carboxyrhodamine110 dye (cRh110, λex = 480 nm; λem = 520 nm) allowing for a sensitive and fast (in-gel fluorescence) detection. A Cys residue is introduced between the dye and N-terminus for further modification with thiol-reactive tags. UbiQ-314 is based on Ub with the following mutations: T7D, L8Y, I13R, E34L, Q40N, L69A, and L71A. Residue Lys58 contains a biotin tag which besides detection and binding also serves to increase USP7 selectivity by prohibiting binding of most USPs.
Additional information
| Weight | 0.005 kg |
|---|---|
| aliquot size | |
| Applications | Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling |
| target | |
| source | |
| shipping | |
| purity | |
| molecular weight | |
| storage | Powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
Gjonaj et al. Chem Comm 2019, 55, 5075.
https://pubs.rsc.org/en/content/articlelanding/2019/cc/c9cc00969h
Mandal et al. Angew Chem Int Ed Engl 2021, 60, 7333.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8048964/
Ekkebus, R., et al. On terminal alkynes that can react with active-site cysteine nucleophiles in proteases. J. Am. Chem. Soc. 135, 2867-2870 (2013).
http://www.ncbi.nlm.nih.gov/pubmed/23387960
Sommer et al. Bioorg Med Chem 2013, 21, 2511.
https://pubmed.ncbi.nlm.nih.gov/23535560/