Ac-hISG15prox-VPS

a potent, irreversible and specific activity-based probe for ISG15 proteases

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350.00

Description

UbiQ-311 is a potent, irreversible and specific activity-based probe for ISG15 proteases. It is prepared by total chemical synthesis and based on the proximal part of human ISG15. To improve stability, Cys77 and Cys144 are replaced by Ser. It contains a C-terminal vinyl pentynyl sulfone (VPS) electrophile which allows for post-labeling modification of cross-linked [UbiQ-311]::[ISG15 protease] complexes by using click chemistry with for example biotin-azide.

 

El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055

Basters et al. Nat Struct Mol Biol. 2017, 24, 270.
https://pubmed.ncbi.nlm.nih.gov/28165509/

Hewing et al. Nat Comm 2018, 9, article number 1162.
https://www.nature.com/articles/s41467-018-03511-6