Ac-hISG15prox-VPS

a potent, irreversible and specific activity-based probe for ISG15 proteases

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Description

UbiQ-311 is a potent, irreversible and specific activity-based probe for ISG15 proteases. It is prepared by total chemical synthesis and based on the proximal part of human ISG15 (aa 77-155). To improve stability, Cys77 and Cys144 are replaced by Ser. It contains a C-terminal vinyl pentynyl sulfone (VPS) electrophile which allows for post-labeling modification of cross-linked [UbiQ-311]::[ISG15 protease] complexes by using click chemistry with for example biotin-azide.

 

El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055

Basters et al. Nat Struct Mol Biol. 2017, 24, 270.
https://pubmed.ncbi.nlm.nih.gov/28165509/

Hewing et al. Nat Comm 2018, 9, article number 1162.
https://www.nature.com/articles/s41467-018-03511-6