
Ac-ISG15prox-VPS
a potent, irreversible and specific activity-based probe for ISG15 proteases
Additional information
Weight | 0.05 kg |
---|---|
aliquot size | |
Applications | Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling, DUB activity profiling, Compound library screening, Target inhibitor profiling |
target | |
source | |
shipping | |
purity | |
storage | upon arrival, powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
sample preparation | For detailed sample preparation see product sheet. |
regulatory statement |
€350.00
- Description
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Description
UbiQ-262 is a potent, irreversible and specific activity-based probe for ISG15 proteases. It is prepared by total chemical synthesis and based on the proximal part of mouse ISG15 (aa 77-155). To improve stability, Cys77 and Cys144 are replaced by Ser. It contains a C-terminal vinyl pentynyl sulfone (VPS) electrophile which allows for post-labeling modification of cross-linked [UbiQ-237]::[ISG15 protease] complexes by using click chemistry with for example biotin-azide.
Additional information
Weight | 0.05 kg |
---|---|
aliquot size | |
Applications | Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling, DUB activity profiling, Compound library screening, Target inhibitor profiling |
target | |
source | |
shipping | |
purity | |
storage | upon arrival, powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
sample preparation | For detailed sample preparation see product sheet. |
regulatory statement |