His6-Ahx-Ahx-SUMO2-VPS

a potent, irreversible and specific activity-based probe for SUMO proteases, labeled with His6 on the N-terminus

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Description

His6-Ahx-Ahx-SUMO2-VPS (UbiQ-237) is an activity-based probe for SUMO proteases that is prepared by total chemical synthesis. It contains a C-terminal vinyl pentynyl sulfone (VPS) electrophile (Figure 1) and an N-terminal His6 sequence which allows for sensitive identification or purification by anti-His6 antibodies and/or anti-His6-agarose. The VPS electrophile allows for post-labeling modification of cross-linked UbiQ-237::SENP complexes by using click chemistry with for example biotin-azide.

 

Albrow et al. Chem Biol 2011, 18, 722.
https://www.ncbi.nlm.nih.gov/pubmed/21700208

Mendes et al. Biochim Biophys Acta – Mol Cell Res 2016, 1863, 139.
https://www.ncbi.nlm.nih.gov/pubmed/26522917