HA-Ahx-Ahx-Ub-VPA

an activity-based probe for deubiquitinating enzymes (DUBs), labeled with HA on the N-terminus

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code UbiQ-219 Category Tags , ,
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300.00

Description

HA-Ahx-Ahx-Ub-VPA (UbiQ-219) is an activity-based probe for deubiquitinating enzymes (DUBs). It is labelled on the N-terminus with the HA peptide sequence (YPYDVPDYA) derived from the influenza hemagglutinin protein and allows for the sensitive identification or purification of DUBs by anti-HA antibodies and/or anti-HA-agarose. The HA tag is separated from the N-terminus by two 6-aminohexanoic acid (Ahx) linkers for efficient recognition of the tag. The alkyne group in the vinyl pentynyl amide warhead allows for further (post-labelling) modification by using click chemistry.

El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055

Borodovsky, A., et al. A novel active site-directed probe specific for deubiquitylating enzymes reveals proteasome association of USP14. EMBO J. 20, 5187-5196 (2001).
http://www.ncbi.nlm.nih.gov/pubmed/11566882

Borodovsky, A., et al. Chemistry-based functional proteomics reveals novel members of the deubiquitinating enzyme family. Chem. Biol. 9, 1149-1159 (2002).
http://www.ncbi.nlm.nih.gov/pubmed/12401499

de Jong, A., et al. Ubiquitin-based probes prepared by total synthesis to profile the activity of deubiquitinating enzymes. ChemBiochem 13, 2251-2258 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/23011887

http://www.ncbi.nlm.nih.gov/pubmed/23011887