Biotin-Ahx-Ub-VS

Name: Biotin-Ahx-Ub-VS
SKU: UbiQ-188
Availability: InStock

activity-based probe for deubiquitinating enzymes (DUBs), labeled with biotin on the N-terminus

productsheet

code UbiQ-188 Category activity-based probes Tags biotin-Ub-VS, DUB probe, ubiquitin, vinyl sulfone, VS probe

product UbiQ-188 Category activity-based probes Tags biotin-Ub-VS, DUB probe, ubiquitin, vinyl sulfone, VS probe

Additional information

Weight	0.05 kg
aliquot size	50 µg
bulk	1 mg
Applications	Crystallization, Purification, Western Blot, Phenotypic protein profiling, DUB activity profiling
target	DUB
source	human sequence, synthetic
shipping	lyophilized powder
purity	≥95% by RP-HPLC and SDS-PAGE
storage	upon arrival, powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles.
sample preparation	For detailed sample preparation see product sheet.
regulatory statement	For laboratory research use only, not for use in humans.

€250.00

Description
Additional information
references

Description

UbiQ-188 is an activity-based probe for deubiquitinating enzymes (DUBs) based on ubiquitin with a C-terminal electrophilic vinyl sulfone (VS) group. It is labeled on the N-terminus with a biotin and an aminohexanoic acid (Ahx) linker is introduced to create extra space between the biotin and Ub protein for efficient access of biotin binding entities. To eliminate Met1 oxidation, Met1 is replaced by norleucine, a well validated Met mimic. UbiQ-188 can be used for proteomics, activity profiling experiments and determining DUB inhibitor specificity.

Additional information

Weight	0.05 kg
aliquot size	50 µg
bulk	1 mg
Applications	Crystallization, Purification, Western Blot, Phenotypic protein profiling, DUB activity profiling
target	DUB
source	human sequence, synthetic
shipping	lyophilized powder
purity	≥95% by RP-HPLC and SDS-PAGE
storage	upon arrival, powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles.
sample preparation	For detailed sample preparation see product sheet.
regulatory statement	For laboratory research use only, not for use in humans.

Galardy, P., et al. Mechanism-based proteomics tools based on ubiquitin and ubiquitin-like proteins: crystallography, activity profiling, and protease identification. Methods in Enzymology. 399, 120-131 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/16338352

de Jong, A., et al. Ubiquitin-based probes prepared by total synthesis to profile the activity of deubiquitinating enzymes. ChemBiochem 13, 2251-2258 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/23011887

Wrigley, J.D. et al. Enzymatic characterisation of USP7 deubiquitinating activity and inhibition. Cell Biochem. Biophys. 60, 99-111 (2011)
http://www.ncbi.nlm.nih.gov/pubmed/21468692