Biotin-Ahx-Ub-VS
a potent and specific inhibitor of deubiquitinating enzymes (DUBs), labeled with biotin on the N-terminus
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| bulk | |
| Applications | Crystallization, Purification, Western Blot, Phenotypic protein profiling, DUB activity profiling |
| target | |
| source | |
| shipping | |
| purity | |
| storage | upon arrival, powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
€250.00
- Description
- Additional information
- references
Description
Biotin-Ahx-Ub-VS (UbiQ-188) is a potent and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic vinyl sulfone (VS) group. UbiQ-188 can be used for activity profiling experiments and determining DUB inhibitor specificity. It is labeled on the N-terminus with a biotin and an aminohexanoic acid (Ahx) linker is introduced to create extra space between the biotin and Ub protein for efficient access of biotin binding entities. To eliminate Met1 oxidation, Met1 is replaced by norleucine, a well validated Met mimic.
Additional information
| Weight | 0.05 kg |
|---|---|
| aliquot size | |
| bulk | |
| Applications | Crystallization, Purification, Western Blot, Phenotypic protein profiling, DUB activity profiling |
| target | |
| source | |
| shipping | |
| purity | |
| storage | upon arrival, powder at −20°C; buffered solution at −80°C. Please avoid multiple freeze/thaw cycles. |
| sample preparation | For detailed sample preparation see product sheet. |
| regulatory statement |
Galardy, P., et al. Mechanism-based proteomics tools based on ubiquitin and ubiquitin-like proteins: crystallography, activity profiling, and protease identification. Methods in Enzymology. 399, 120-131 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/16338352
de Jong, A., et al. Ubiquitin-based probes prepared by total synthesis to profile the activity of deubiquitinating enzymes. ChemBiochem 13, 2251-2258 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/23011887
Wrigley, J.D. et al. Enzymatic characterisation of USP7 deubiquitinating activity and inhibition. Cell Biochem. Biophys. 60, 99-111 (2011)
http://www.ncbi.nlm.nih.gov/pubmed/21468692