HA-Ahx-Ahx-Ub-VS

a potent and specific inhibitor of deubiquitinating enzymes (DUBs), labeled with HA on the N-terminus

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Weight 0.05 kg
aliquot size

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Description

HA-Ahx-Ahx-Ub-VS (UbiQ-187) is a potent and specific inhibitor of deubiquitinating enzymes (DUBs) based on a C-terminal electrophilic vinyl sulfone (VS) group. UbiQ-187 can be used for activity profiling experiments and determining DUB inhibitor specificity. UbiQ-187 contains an N-terminal HA-tag (YPYDVPDYA), which is a peptide sequence derived from the influenza hemagglutinin protein and allows for the sensitive identification or purification by anti-HA antibodies and/or anti-HA-agarose. The HA tag is separated from the Ub N-terminus by two aminohexanoic acid (Ahx) linkers for efficient recognition of the tag. To eliminate Met1 oxidation, Met1 is replaced by norleucine, a well validated Met mimic.

Additional information

Weight 0.05 kg
aliquot size

Applications

, , , ,

target

source

shipping

purity

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Galardy, P., et al. Mechanism-based proteomics tools based on ubiquitin and ubiquitin-like proteins: crystallography, activity profiling, and protease identification. Methods in Enzymology. 399, 120-131 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/16338352

de Jong, A., et al. Ubiquitin-based probes prepared by total synthesis to profile the activity of deubiquitinating enzymes. ChemBiochem 13, 2251-2258 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/23011887

Wrigley, J.D. et al. Enzymatic characterisation of USP7 deubiquitinating activity and inhibition. Cell Biochem. Biophys. 60, 99-111 (2011)
http://www.ncbi.nlm.nih.gov/pubmed/21468692