Biotin-Ahx-H2A(112-129) K119Ub (numbering without Met1)

an H2A(112-129) peptide which is modified at K119* via a native isopeptide bond with ubiquitin and modified on the N-terminus with biotin
(numbering without Met1)

productsheet

Additional information

Weight 0.05 kg
aliquot size

Applications

, , , , ,

target

,

source

human sequence, synthetic

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Clear

300.00

Description

UbiQ-185 is an H2A(112-129) peptide which is modified at K119* via a native isopeptide bond with ubiquitin (Ub) and modified on the N-terminus with biotin. An aminohexanoic acid (Ahx) linker is used to create extra space between the biotin and H2A peptide for efficient access of biotin binding entities. It can be used as a substrate for ubiquitin proteases, to investigate mechanism of binding and recognition by proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs (UIMs) and as antigen for immunizations.
* numbering without Met1

Additional information

Weight 0.05 kg
aliquot size

Applications

, , , , ,

target

,

source

human sequence, synthetic

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055