Biotin-Ahx-EGTKAVTK(Ub)YTSSK

an H2B(113-125) polypeptide, labeled with biotin on the N-terminus and monoubiquitinated at K120 via a native isopeptide bond

productsheet

code UbiQ-184 Category
product UbiQ-184 Category

Additional information

Weight 0.05 kg
aliquot size

Applications

target

source

human sequence, synthetic

shipping

purity

molecular weight

storage

sample preparation

• dissolve the powder in as little DMSO as possible (e.g. 20 – 40 mg/mL)
• add the DMSO stock slowly to milliQ (please note the order of addition)
• buffer the aq. solution as desired (e.g. 50 mM HEPES pH 8, 100 mM NaCl)
• for example, a final buffered stock of 0.5 mg/mL will contain 1.25 vol% DMSO when prepared from a 40 mg/mL DMSO stock.

regulatory statement

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300.00

Description

UbiQ-184 is an N-terminal biotinylated H2B(113-125) peptide which is monoubiquitinated at K120
via a native isopeptide bond. An aminohexanoic acid (Ahx) linker is used to create extra space between the
biotin and H2B peptide for efficient access of biotin binding entities. This product is formed by chemical
ligation.

Additional information

Weight 0.05 kg
aliquot size

Applications

target

source

human sequence, synthetic

shipping

purity

molecular weight

storage

sample preparation

• dissolve the powder in as little DMSO as possible (e.g. 20 – 40 mg/mL)
• add the DMSO stock slowly to milliQ (please note the order of addition)
• buffer the aq. solution as desired (e.g. 50 mM HEPES pH 8, 100 mM NaCl)
• for example, a final buffered stock of 0.5 mg/mL will contain 1.25 vol% DMSO when prepared from a 40 mg/mL DMSO stock.

regulatory statement

El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055