Biotin-Ahx-H2B(106-125) K120 Ub

an H2B(106-125) polypeptide, labeled with biotin on the N-terminus and monoubiquitinated at K120 via a native isopeptide bond

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Additional information

Weight 0.05 kg
aliquot size

Applications

, , , , ,

target

source

human sequence, synthetic

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molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

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Description

UbiQ-150 is a H2B(106-125) polypeptide which is labeled on the N-terminus with biotin (separated by an aminohexanoic acid linker) and monoubiquitinated at K120 via a native isopeptide bond. It can be used as a substrate for deubiquitylases to investigate mechanism of binding and recognition by proteins that contain ubiquitin-associated domains or ubiquitin-interacting motifs (UIMs) and as antigen for immunizations. This product is formed by chemical ligation.

Additional information

Weight 0.05 kg
aliquot size

Applications

, , , , ,

target

source

human sequence, synthetic

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

F. El Oualid et al.
El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055

Faesen et al.
Faesen, A.C., et al. The Differential Modulation of USP Activity by Internal Regulatory Domains, Interactors and Eight Ubiquitin Chain Types. Chem. Biol. 18, 1550-1561 (2011).
http://www.ncbi.nlm.nih.gov/pubmed/22195557

I. Dikic et al.
Dikic, I., et al. Ubiquitin-binding domains – from structures to functions. Nat. Rev. Mol. Cell. Biol. 10, 659-671 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/19773779

J. D. F. Licchesi et al.
Licchesi, J.D., et al. An ankyrin-repeat ubiquitin-binding domain determines TRABID’s specificity for atypical ubiquitin chains. Nat. Struct. Mol. Biol. 19, 62-71 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/22157957