5-carboxyRh110-Ub-Dha

an activity based probe for Ub E1, E2 and (HECT/RBR) E3 ligases, labeled with cRh110 on the N-terminus

productsheet

code UbiQ-131 Category
product UbiQ-131 Category

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Weight 0.005 kg
aliquot size

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Description

UbiQ-131 is a first of its kind activity based probe for Ub E1, E2 and (HECT/RBR) E3 ligases [Mulder et al. 2016]. It is based on the Ub sequence in which the C-terminal Gly76 has been replaced by a dehydroalanine (Dha) residue. The N-terminus is labeled with the green fluorescent 5-carboxyrhodamine110 dye (cRh110). It has been prepared by total chemical synthesis and is therefore well-defined in terms of dye site.

UbiQ-131 is processed in a native manner by Ub E1, E2 and (HECT/RBR) E3 ligases and during this process it forms an electrophilic intermediate that can react with the active site Cys residue of the E1, E2 and (HECT/RBR) E3 enzyme, thereby creating a covalent bond (see figure).

Additional information

Weight 0.005 kg
aliquot size

Applications

, ,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Mulder et al., A cascading activity-based probe sequentially targets E1–E2–E3 ubiquitin enzymes. Nature Chem Bio (2016) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5108872/