Ub-Rh110MP

Name: Ub-Rh110MP
SKU: UbiQ-126
Availability: InStock

a quenched, fluorescent substrate for ubiquitin proteases, releasing a brighter Rh110MP dye upon DUB cleavage than UbRh110Gly

productsheet

code UbiQ-126 Category activity assay reagents Tag Ub-Rh110MP

product UbiQ-126 Category activity assay reagents Tag Ub-Rh110MP

Additional information

Weight	0.005 kg
aliquot size	50 µg
bulk	1 mg, 5 mg, 10 mg
Applications	DUB activity profiling, Compound library screening
target	DUB
source	human sequence, synthetic
shipping	lyophilized powder
purity	≥95% by HPLC and cation-chromatograpgy
molecular weight	8.99 kDa
storage	upon arrival powder at −20°C, solution at −80°C. Protect from light and avoid multiple freeze/thaw cycles.
sample preparation	For detailed sample preparation see product sheet.
regulatory statement	For laboratory research use only, not for use in humans.

€250.00

Description
Additional information
references

Description

Ub-Rh110MP (UbiQ-126) is a quenched, fluorescent substrate for deubiquitylases (DUBs). Cleavage of the amide bond between Gly76 of ubiquitin and the Rhodamine110 moiety releases the highly fluorescent Rh110-morpholinecarbonyl which exhibits a higher fluorescence intensity than the classical Rh110Gly fluorophore of Ub-Rh110Gly (UbiQ-002). UbiQ-126 is prepared by total chemical synthesis.

Additional information

Weight	0.005 kg
aliquot size	50 µg
bulk	1 mg, 5 mg, 10 mg
Applications	DUB activity profiling, Compound library screening
target	DUB
source	human sequence, synthetic
shipping	lyophilized powder
purity	≥95% by HPLC and cation-chromatograpgy
molecular weight	8.99 kDa
storage	upon arrival powder at −20°C, solution at −80°C. Protect from light and avoid multiple freeze/thaw cycles.
sample preparation	For detailed sample preparation see product sheet.
regulatory statement	For laboratory research use only, not for use in humans.

Hassiepin, U., et al. A sensitive fluorescence intensity assay for deubiquitinating proteases using ubiquitin-rhodamine 110-glycine as substrate. Anal. Biochem. 371, 201-207 (2007).
http://www.ncbi.nlm.nih.gov/pubmed/17869210

El Oualid, F., et al. Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin. Angewandte Chemie Int. Ed. 49, 10149-10153 (2010).
http://www.ncbi.nlm.nih.gov/pubmed/21117055

Lavis, L.D., et al. Fluorogenic label for biomolecular imaging. ACS Chem. Biol. 1, 252-260 (2006).
http://www.ncbi.nlm.nih.gov/pubmed/17163679

Terentyeva, T.G., et al. Morpholinecarbonyl-Rhodamine 110 based substrates for the determination of protease activity with accurate kinetic parameters. Bioconj. Chem. 22, 1932-1938 (2011).
http://www.ncbi.nlm.nih.gov/pubmed/21905728