5-carboxyRh110-Nedd8-Dha

an activity based probe for Ub E1, E2 and (HECT/RBR) E3 ligases, labeled with cRh110 on the N-terminus

productsheet

code UbiQ-122 Category
product UbiQ-122 Category

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Weight 0.005 kg
aliquot size

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Description

UbiQ-122 is a first of its kind activity based probe for Nedd8 E1, E2 and E3 ligases [Mulder et al. 2016]. It is based on the Nedd8 sequence in which the C-terminal Gly76 has been replaced by a dehydroalanine (Dha) residue. The N-terminus is labeled with the green fluorescent 5-carboxyrhodamine110 dye (cRh110). It has been prepared by total chemical synthesis and is therefore well-defined in terms of dye site.

UbiQ-122 is processed in a native manner by E1, E2 and E3 ligases and during this process, it forms an electrophilic intermediate that can react with the active site Cys residue of the E1, E2 and E3 enzyme, thereby creating a covalent bond (see figure)

Additional information

Weight 0.005 kg
aliquot size

Applications

, ,

target

,

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Mulder et al., A cascading activity-based probe sequentially targets E1–E2–E3 ubiquitin enzymes. Nature Chem Bio (2016) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5108872/