Biotin-Ahx-Ub(1-75)-Dha-Ub
an activity-based probe based on linear diubiquitin which targets the deubiquitinating enzyme (DUB) OTULIN
Additional information
Weight | 0.05 kg |
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aliquot size | |
Applications | Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling |
target | |
source | |
shipping | |
purity | |
molecular weight | |
storage | |
sample preparation | For detailed sample preparation see product sheet. |
regulatory statement |
€350.00
- Description
- Additional information
- references
Description
UbiQ-121 is an activity-based probe based on linear diubiquitin which targets the deubiquitinating enzyme (DUB) OTULIN. In UbiQ-121, the Gly76-Met1 dipeptide of linear diUb is replaced by a dehydroalanine-Met (Dha-Met) dipeptide. The N-terminus of the distal Ub is labeled with biotin; an aminohexanoic acid (Ahx) linker is used to create extra space for efficient access of biotin binding entities. UbiQ-121 has been prepared by total chemical synthesis and is therefore well-defined in terms of biotinylation site. UbiQ-121 reacts with OTULIN in a covalent manner without being cleaved into monoUb, indicating correct positioning of the Dha electrophile for reacting with the nucleophilic active site cysteine of OTULIN. The DUB activity based probe can be used for activity profiling experiments and structural studies. Please note that the native distance between the proximal and distal Ub is preserved.
Additional information
Weight | 0.05 kg |
---|---|
aliquot size | |
Applications | Crystallization, Pull down, Purification, Western Blot, MS, NMR, Phenotypic protein profiling |
target | |
source | |
shipping | |
purity | |
molecular weight | |
storage | |
sample preparation | For detailed sample preparation see product sheet. |
regulatory statement |
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http://www.ncbi.nlm.nih.gov/pubmed/24364494
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http://www.ncbi.nlm.nih.gov/pubmed/24225431
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http://www.ncbi.nlm.nih.gov/pubmed/22689415
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http://www.ncbi.nlm.nih.gov/pubmed/24290882
Weber, A., et al. A Linear Diubiquitin-Based Probe for Efficient and Selective Detection of the Deubiquitinating Enzyme OTULIN Cell Chem Biol. 2017 DOI: http://dx.doi.org/10.1016/j.chembiol.2017.08.006
https://www.ncbi.nlm.nih.gov/pubmed/28919039