SUMO3-Dha

an activity based probe for the SUMO ligation pathway

productsheet

Additional information

Weight 0.005 kg
wholesale

,

catalogue

Applications

,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Clear
View cart

300.00

Description

SUMO3-Dha (UbiQ-118) (Dha= dehydroalanine) is a first of its kind activity based probe for the SUMO ligation pathway. It is based on the SUMO3 sequence in which the C-terminal Gly has been replaced by a dehydroalanine residue. The native Cys47 has been mutated to a Ser residue. UbiQ-118 is processed in a native manner by SUMO E1, E2 and E3 ligases and during this process, it forms an electrophilic intermediate that can react with the active site Cys residue of the E1, E2 and E3 enzymes, thereby creating a covalent bond (see figure).

Additional information

Weight 0.005 kg
wholesale

,

catalogue

Applications

,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Mulder et al., A cascading activity-based probe sequentially targets E1–E2–E3 ubiquitin enzymes. Nature Chem Bio (2016) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5108872/