SUMO1-Dha

an activity based probe for the SUMO ligation pathway

productsheet

code UbiQ-116 Category Tag
product UbiQ-116 Category Tag

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Weight 0.005 kg
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300.00

Description

SUMO1-Dha (UbiQ-116) (Dha= dehydroalanine) is a first of its kind activity based probe for the SUMO ligation pathway. It is based on the SUMO1 sequence in which the C-terminal Gly has been replaced by a dehydroalanine residue. The native Cys52 has been mutated to a Ser residue. UbiQ-116 is processed in a native manner by SUMO E1, E2 and E3 ligases and during this process, it forms an electrophilic intermediate that can react with the active site Cys residue of the E1, E2 and E3 enzymes, thereby creating a covalent bond (see figure).

Additional information

Weight 0.005 kg
catalogue

wholesale

Applications

,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Mulder et al., A cascading activity-based probe sequentially targets E1–E2–E3 ubiquitin enzymes. Nature Chem Bio (2016) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5108872/