K63 diUb PA

a potent, irreversible and specific inhibitor based on K63 linked diUb targeting the S2 pocket of deubiquitinating enzymes (DUBs)

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Weight 0.005 kg
aliquot size

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Description

K63 diUb PA (UbiQ-114) is a potent, irreversible and specific inhibitor based on K63 linked diUb that targets the S2 pocket of deubiquitinating enzymes (DUBs). The C-terminus of the proximal Ub is equiped with the propargylamide (PA) electrophile and the isopeptide bond between the two Ub proteins is replaced by a DUB resistant mimic.

This DUB activity based probe can be used for activity profiling experiments and structural studies. Please note the native distance between the proximal and distal Ub is preserved as much as possible. UbiQ-114 has been prepared by total chemical synthesis.

Additional information

Weight 0.005 kg
aliquot size

Applications

, , , ,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Galardy, P., et al. Mechanism-based proteomics tools based on ubiquitin and ubiquitin-like proteins: crystallography, activity profiling, and protease identification. Methods in Enzymology. 399, 120-131 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/16338352

Ekkebus, R., et al. On terminal alkynes that can react with active-site cysteine nucleophiles in proteases. J. Am. Chem. Soc. 135, 2867-2870 (2013).
http://www.ncbi.nlm.nih.gov/pubmed/23387960