Cy5-Ub-PA

a potent and specific inhibitor of deubiquitylating enzymes (DUBs), labeled with a Cy5 dye on the N-terminus

productsheet

product UbiQ-072 Category Tags , , ,

Additional information

Weight 0.005 kg
aliquot size

Applications

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source

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purity

molecular weight

storage

Powder at −20°C; solution at −80°C. Please avoid multiple freeze/thaw cycles.

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

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250.00

Description

UbiQ-072 (Cy5-Ub-PA) is a potent and specific inhibitor of deubiquitylating enzymes (DUBs). This activity probe is labeled on the N-terminus with a Cy5 dye (Cy5, exc 625-650 nm, emi 670 nm) and can be used for activity profiling experiments and determining DUB inhibitor specificity.

It has three unique capabilities:

  1. it forms a covalent linkage with (the active site Cys residue of) a DUB that can be cleaved by acid treatment (5% aq. TFA), allowing for proteomic analyses;
  2. it targets all three major DUB families: UCH, USP and OTU;
  3. the TAMRA label allows detection of DUB labeling by direct in-gel fluorescence. This is a less time-consuming and more sensitive read-out than western-blotting

Finally, cross-reactivity of antibodies can lead to background labeling, something that is not observed with Cy5-Ub-PA (UbiQ-072).

Additional information

Weight 0.005 kg
aliquot size

Applications

, , , ,

target

source

shipping

purity

molecular weight

storage

Powder at −20°C; solution at −80°C. Please avoid multiple freeze/thaw cycles.

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

de Jong et al.
de Jong, A., et al. Ubiquitin-based probes prepared by total synthesis to profile the activity of deubiquitinating enzymes. ChemBiochem 13, 2251-2258 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/23011887

Altun et al.
Altun, M., et al. Activity-based chemical proteomics accelerates inhibitor development for deubiquitylating enzymes. Chem. Biol. 18, 1401-1412 (2011).
http://www.ncbi.nlm.nih.gov/pubmed/22118674

Ekkebus et al.
Ekkebus, R., et al. On terminal alkynes that can react with active-site cysteine nucleophiles in proteases. J. Am. Chem. Soc. 135, 2867-2870 (2013).
http://www.ncbi.nlm.nih.gov/pubmed/23387960

Sommer et al.
Sommer, S., et al. Covalent inhibition of SUMO and ubiquitin-specific cysteine proteases by an in situ thiol-alkyne addition. Bioorg. Med. Chem. 21, 2511-2517 (2013).
http://www.ncbi.nlm.nih.gov/pubmed/23535560