Cy5-Ub-VME

a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs), labeled with a Cy5 dye on the N-terminus

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product UbiQ-071 Category Tags , ,

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Weight 0.005 kg
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Custom Aliquot size

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Description

Cy5-Ub-VME (UbiQ-071) is a potent, irreversible and specific inhibitor of deubiquitylating enzymes (DUBs), which is labeled on the N-terminus with a Cy5 dye (Cy5, exc 625-650 nm, abs 670 nm). This ubiquitin-based activity probe can be used for activity profiling experiments and the control of DUB inhibitor specificity. Whereas the first-generation activity probes (such as HA tagged Ub-VME) require immunoblotting for detection.

This probe allows for detection of DUB labeling by in-gel fluorescence. This direct and more sensitive read-out gives more distinct labeling patterns than can be achieved by immunoblotting. In addition, cross-reactivity of antibodies can lead to background labeling, something that is not observed with UbiQ-071.

Additional information

Weight 0.005 kg
aliquot size

Custom Aliquot size

Applications

, , , ,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Misaghi, S., et al. Structure of the Ubiquitin Hydrolase UCH-L3 Complexed with a Suicide Substrate. J. Biol. Chem. 280, 1512-1520 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/15531586

de Jong, A., et al. Ubiquitin-based probes prepared by total synthesis to profile the activity of deubiquitinating enzymes. ChemBiochem 13, 2251-2258 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/23011887

Altun, M., et al. Activity-based chemical proteomics accelerates inhibitor development for deubiquitylating enzymes. Chem. Biol. 18, 1401-1412 (2011).
http://www.ncbi.nlm.nih.gov/pubmed/22118674

Wrigley, J.D. et al. Enzymatic characterisation of USP7 deubiquitinating activity and inhibition. Cell Biochem. Biophys. 60, 99-111 (2011)
http://www.ncbi.nlm.nih.gov/pubmed/21468692