Ub-PA

a potent and specific inhibitor of deubiquitylating enzymes (DUBs)

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code UbiQ-057 Category Tags , , ,
product UbiQ-057 Category Tags , , ,

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Weight 0.005 kg
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bulk

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Description

Ubiquitin-propargylamide is a potent and specific inhibitor of deubiquitylating enzymes (DUBs). This activity probe can be used for activity profiling experiments and determining DUB inhibitor specificity. It has two unique capabilities:

  1. It forms a covalent linkage with (the active site Cys residue of) a DUB that can be cleaved by acid treatment (5% aq. TFA), allowing for proteomic analyses.
  2. It targets all three major DUB families: UCH, USP and OTU.

Additional information

Weight 0.005 kg
aliquot size

bulk

,

Applications

, , , , , ,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Galardy et al.
Galardy, P., et al. Mechanism-based proteomics tools based on ubiquitin and ubiquitin-like proteins: crystallography, activity profiling, and protease identification. Methods in Enzymology. 399, 120-131 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/16338352

Ekkebus et al.
Ekkebus, R., et al. On terminal alkynes that can react with active-site cysteine nucleophiles in proteases. J. Am. Chem. Soc. 135, 2867-2870 (2013).
http://www.ncbi.nlm.nih.gov/pubmed/23387960

Sommer et al.
Sommer, S., et al. Covalent inhibition of SUMO and ubiquitin-specific cysteine proteases by an in situ thiol-alkyne addition. Bioorg. Med. Chem. 21, 2511-2517 (2013).
http://www.ncbi.nlm.nih.gov/pubmed/23535560