Ub-Ub(22-35)-FP K29 linked

a class II ubiquitinated peptide fluorescence polarization assay reagent based on the peptide sequence 22 – 35 of ubiquitin

productsheet

code UbiQ-046 Category
product UbiQ-046 Category

Additional information

Weight 0.005 kg
aliquot size

Applications

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target

source

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purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

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300.00

Description

UbiQ-046 is a class II ubiquitinated peptide fluorescence polarization assay reagent based on the peptide sequence 22 – 35 of ubiquitin.
The peptide is modified on the N-terminus with a 5-carboxytetramethylrhodamine and conjugated at Lys29 to Ub via a native isopeptide bond.
See reference 5 in the product sheet (open access) for full experimental and analytical details.

Additional information

Weight 0.005 kg
aliquot size

Applications

,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.

regulatory statement

Tirat, A., et al. Synthesis and characterization of fluorescent ubiquitin derivatives as highly sensitive substrates for the deubiquitinating enzymes UCH-L3 and USP-2. Anal. Biochem. 343, 244-255 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/15963938

Huang, X. & Aulabaugh A. Application of fluorescence polarization in HTS assays. Methods in Molecular Biology 565, 127-143 (2009).
http://www.ncbi.nlm.nih.gov/pubmed/19551360

Levine, L.M., et al. Measurement of specific protease activity utilizing fluorescence polarization. Anal. Biochem. 247, 83-88 (1997).
http://www.ncbi.nlm.nih.gov/pubmed/9126375

Geurink, P.P., et al. A general chemical ligation approach towards isopeptide-linked ubiquitin and ubiquitin-like assay reagents. ChemBiochem 13, 293-297 (2012).
http://www.ncbi.nlm.nih.gov/pubmed/22213387

Faesen, A.C., et al. The Differential Modulation of USP Activity by Internal Regulatory Domains, Interactors and Eight Ubiquitin Chain Types. Chem. Biol. 18, 1550-1561 (2011).
http://www.ncbi.nlm.nih.gov/pubmed/22195557