5-TAMRA-Lys(Ub)-Gly-OH (Ub-FP)

a fluorescence polarization assay reagent for deubiquitinating enzymes (DUBs) based on 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly dipeptide that is linked to ubiquitin via a native isopeptide linkage
productsheet
code UbiQ-012 Category Tags , , , ,
product UbiQ-012 Category Tags , , , ,

Additional information

Weight 0.005 kg
aliquot size

Applications

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target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.
regulatory statement

€270.00

Description

UbiQ-012 (Ub-FP, 5-TAMRA-Lys(Ub)-Gly-OH) is a fluorescence polarization assay reagent for deubiquitinating enzymes (DUBs). It is based on a 5-carboxytetramethylrhodamine (TAMRA, exc 550 nm, emi 590 nm) modified Lys-Gly dipeptide that is linked to ubiquitin (Ub) via a native isopeptide linkage.

Additional information

Weight 0.005 kg
aliquot size

Applications

, ,

target

source

shipping

purity

molecular weight

storage

sample preparation

For detailed sample preparation see product sheet.
regulatory statement

  1. Tirat et al. Synthesis and characterization of fluorescent ubiquitin derivatives as highly sensitive substrates for the deubiquitinating enzymes UCH-L3 and USP-2. Analytical Biochem 2005, 343, 244: http://www.ncbi.nlm.nih.gov/pubmed/15963938
  2. Huang and Aulabaugh, Application of fluorescence polarization in HTS assays. Methods in Molecular Biology 2009, 565, 127: http://www.ncbi.nlm.nih.gov/pubmed/19551360
  3. Levine et al. Measurement of specific protease activity utilizing fluorescence polarization. Analytical Biochem 1997, 247, 83: http://www.ncbi.nlm.nih.gov/pubmed/9126375
  4. Geurink et al. A general chemical ligation approach towards isopeptide-linked ubiquitin and ubiquitin-like assay reagents. ChemBiochem 2012, 13, 293: http://www.ncbi.nlm.nih.gov/pubmed/22213387
Ubiquitin FP (Ub-FP, UbiQ-012) is a fluorescence polarization assay reagent for deubiquitinating enzymes (DUBs). It is based on a Lys-Gly dipeptide that is modified on the N-terminus with a 5-carboxytetramethylrhodamine (TAMRA) dye and monoubiquitinated on the Lys residue via a native isopeptide. Our FP reagent design also allows for the addition of more and specific substrate context by introducing 10 to 15 amino acids long polypeptides (e.g. our class II FP reagents). Compared to quenched assay reagents, Ub-FP assay reagents have two unique characteristics. First, they contain a native isopeptide bond, and second, they allow for adding more substrate context than ubiquitin alone.
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