Ub-FP

Name: Ub-FP
SKU: UbiQ-012
Availability: InStock

a fluorescence polarization assay reagent based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to ubiquitin via a native isopeptide bond with the lysine side-chain

productsheet

code UbiQ-012 Category activity assay reagents Tags assay, compound library screening, DUB activity assay, fluorescence polarization, ubiquitin

product UbiQ-012 Category activity assay reagents Tags assay, compound library screening, DUB activity assay, fluorescence polarization, ubiquitin

Additional information

Weight	0.005 kg
aliquot size	50 µg
Applications	DUB activity profiling, Compound library screening, Target inhibitor profiling
target	DUB
source	human sequence, semi-synthetic
shipping	lyophilized powder
purity	>95%
molecular weight	9.16 kDa
storage	upon arrival, powder at −20°C; solution at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles.
sample preparation	For detailed sample preparation see product sheet.
regulatory statement	For laboratory research use only, not for use in humans.

€250.00

Description
Additional information
references
ubiquitin FP

Description

UbiQ-012 (Ub-FP) is a fluorescence polarization assay reagent which is based on a 5-carboxytetramethylrhodamine (TAMRA) modified Lys-Gly sequence that is linked to ubiquitin via a native isopeptide bond with the lysine side-chain. Typical substrate concentrations range from 10−100 nM. DUB concentrations can range from 0.01-10 nM but depend on specific assay conditions and method of detection.

Additional information

Weight	0.005 kg
aliquot size	50 µg
Applications	DUB activity profiling, Compound library screening, Target inhibitor profiling
target	DUB
source	human sequence, semi-synthetic
shipping	lyophilized powder
purity	>95%
molecular weight	9.16 kDa
storage	upon arrival, powder at −20°C; solution at −80°C. Protect from light. Please avoid multiple freeze/thaw cycles.
sample preparation	For detailed sample preparation see product sheet.
regulatory statement	For laboratory research use only, not for use in humans.

Tirat, A., et al. Synthesis and characterization of fluorescent ubiquitin derivatives as highly sensitive substrates for the deubiquitinating enzymes UCH-L3 and USP-2. Anal. Biochem. 343, 244-255 (2005).
http://www.ncbi.nlm.nih.gov/pubmed/15963938

Huang, X. & Aulabaugh A. Application of fluorescence polarization in HTS assays. Methods in Molecular Biology 565, 127-143 (2009). http://www.ncbi.nlm.nih.gov/pubmed/19551360

Levine, L.M., et al. Measurement of specific protease activity utilizing fluorescence polarization. Anal. Biochem. 247, 83-88 (1997). http://www.ncbi.nlm.nih.gov/pubmed/9126375

Geurink, P.P., et al. A general chemical ligation approach towards isopeptide-linked ubiquitin and ubiquitin-like assay reagents. ChemBiochem 13, 293-297 (2012). http://www.ncbi.nlm.nih.gov/pubmed/22213387

Ubiquitin FP (Ub-FP, UbiQ-012) is a fluorescence polarization assay reagent for deubiquitinating enzymes (DUBs). It is based on a Lys-Gly dipeptide that is modified on the N-terminus with a 5-carboxytetramethylrhodamine (TAMRA) dye and monoubiquitinated on the Lys residue via a native isopeptide. Our FP reagent design also allows for the addition of more and specific substrate context by introducing 10 to 15 amino acids long polypeptides (e.g. our class II FP reagents). Compared to quenched assay reagents, Ub-FP assay reagents have two unique characteristics. First, they contain a native isopeptide bond, and second, they allow for adding more substrate context than ubiquitin alone.

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