Activity-based probes have proven to be efficient tools for structural biology. Over the years a wide range of deubiquitinating and ubiquitinating enzymes have been elucidated using these probes. Here we give you an (in-comprehensive) overview of the most recent crystallizations.

• E2 enzyme: UBE2D3, probe: UbiQ-101 Ub-Dha (accession number 5IFR1)
• DUB MINDY-1, probe: UbiQ-057 : Ub-PA (accession number 5JKN, 5JQS)
• DUB UCH-L5, probe: UbiQ-057 : Ub-PA (accession number 4UEL, 4UF6)
• DUB USP4, probe: UbiQ-057 : Ub-PA (accession number 2Y6E)
• DUB CCHF, probe: UbiQ-057 : Ub-PA (accession number 3ZNH)
• DUB SARS PLpro, probe: diUb-PA (accession number 5E6J)
• DUB UCH-L1, probe: diUb-PA (accession number 3KW5)
• DUB UCH-L3, probe: diUb-PA (accession number 1XD3, 2WDT)
• DUB MCMV M48, probe: diUb-PA (accession number 2J7Q)

E1-E2-E3 enzyme probe

• UbiQ-101 : Ub-Dha
• UbiQ-105 : Nedd8-Dha

1st generation DUB probes: to target the S1 pocket

• UbiQ-057 : Ub-PA
• UbiQ-005 : Ub-VME
• UbiQ-108 : Ub-VS

2nd generation DUB probes: to target the S1 and S1′ pockets

• UbiQ-081 : K6   diUb-VME
• UbiQ-082 : K11 diUb-VME
• UbiQ-083 : K27 diUb-VME
• UbiQ-084 : K29 diUb-VME
• UbiQ-085 : K33 diUb-VME
• UbiQ-086 : K48 diUb-VME
• UbiQ-087 : K63 diUb-VME

3rd generation DUB probes: to target the S1 and S2 pockets

• UbiQ-114 : K63 diUb-PA

tailor made
A series of proprietary techniques give use structural control on all aspects of our reagents, enabling us to construct reagents that are beyond the reach of any currently available alternative approaches.