Description

UbiQ-072 (Cy5-Ub-PA) is a potent and specific inhibitor of deubiquitylating enzymes (DUBs). This activity probe is labeled on the N-terminus with a Cy5 dye (Cy5, exc 625-650 nm, emi 670 nm) and can be used for activity profiling experiments and determining DUB inhibitor specificity.

It has three unique capabilities:

1. it forms a covalent linkage with (the active site Cys residue of) a DUB that can be cleaved by acid treatment (5% aq. TFA), allowing for proteomic analyses;
2. it targets all three major DUB families: UCH, USP and OTU;
3. the TAMRA label allows detection of DUB labeling by direct in-gel fluorescence. This is a less time-consuming and more sensitive read-out than western-blotting

Finally, cross-reactivity of antibodies can lead to background labeling, something that is not observed with Cy5-Ub-PA (UbiQ-072).